Article Review: Editing genome of Toxoplasma gondii by CRISPR/Cas9 system
نویسندگان
چکیده
It is well known that finding out the genetic information of any organism helps in understanding how performs its metabolism, and knowing extent effect gene modification affects performance these functions. Many techniques have been used to editing genome parasites such as Zinc fingers Transcription activator-like effector nucleases (TALENs), But development clustered regularly interspaced short palindromic repeats/ CRISPR-associated (CRISPR Cas9) system created quantum leap this area, technique relies on identifying portion be modified using a guide from Guide RNAs (gRNA) identifies part very accurately. Not only that, but enzyme present with it called CAS9 works molecular scissor cut DNA at allow required modification.Since 2014, date which first article about use CRISPR/Cas9 Toxoplasma gondii was published, after continued, developed new methods protocols facilitate researchers work, we will attempt paper review some major achievements.
منابع مشابه
Genetic Manipulation of the Toxoplasma gondii Genome by Fosmid Recombineering
UNLABELLED Apicomplexa are obligate intracellular parasites that cause important diseases in humans and animals. Manipulating the pathogen genome is the most direct way to understand the functions of specific genes in parasite development and pathogenesis. In Toxoplasma gondii, nonhomologous recombination is typically highly favored over homologous recombination, a process required for precise ...
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ToxoDB (http://ToxoDB.org) provides a genome resource for the protozoan parasite Toxoplasma gondii. Several sequencing projects devoted to T. gondii have been completed or are in progress: an EST project (http://genome.wustl.edu/est/index.php?toxoplasma=1), a BAC clone end-sequencing project (http://www.sanger.ac.uk/Projects/T_gondii/) and an 8X random shotgun genomic sequencing project (http:/...
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ژورنال
عنوان ژورنال: Ma?alla? ??mi?a? al-anb?r li-l-?ul?m al-?irfa?
سال: 2022
ISSN: ['1991-8941', '2706-6703']
DOI: https://doi.org/10.37652/juaps.2022.172354